Urinary tract infections (UTIs) are a major infection burden globally and antimicrobial resistance can lead to treatment failures as well as upsurge cost of healthcare. Complicated UTIs are always perilous. To know the common isolates and their resistance pattern in urine, 609 samples with suspected UTI patients were studied. Out of 609 samples, 44.1% found positive for the presence of significant bacteriuria. 44.8% of samples of female found positive whereas 43.4% of samples of the male were found positive during the study period. Escherichia coli was the most common isolate followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. All antibiotics found more or less resistant against various organisms which are quite alarming. Most trusted antibiotic against UTI, Nitrofurantoin found 29.3% resistant overall. E. coli found resistance to ampicillin (43%), ampicillin/sulbactam (56%), cefuroxime (86%), levofloxacin (84%), meropenem (15%), nitrofurantoin (7%), tobramycin (60%), and cefazolin (85%). It is advisable to conduct periodic assessments of antibiotic usage and monitoring of resistance pattern locally. In vivo and in vitro difference should be compared and studied in the future.