The virulence of Candida species is contributed by the secretion of enzymes like phospholipase and proteinases as they play a role in invasion of host mucosal epithelia. The samples collected were urine, sputum, pus, and blood. A total of 100 samples that yielded Candida species on culture were studied for Extracellular phospholipase production on in–house prepared Sabouraud’s dextrose egg yolk agar. Out of 100 isolates, 38 showed extracellular phospholipase activity and 62 showed absence or weak phospholipase activity.. This study will help clinicians correlate the virulence of the organism with the severity of the infection in the patient.