Aim: To determine the magnitude of quality indicator in clinical chemistry laboratory tests at pre analytical phase of the assay. Material and method: Hospital based retrospective study, total 236,100 samples were analysed in Department of Biochemistry from inpatient, outpatient and emergency of IPGME & R and SSKM Hospital, West Bengal over a period of one year, April 2018 to March 2019. 10 (ten) IFCC recommended Model Quality Indicator reviewed and frequency of observation analysed. Results: Highest frequency of occurrence monitored in relation to incomplete patient information and inappropriate container and improper proportion (MQI) in all three divisions of patient care service. (average 20–40 samples per 1000 samples).Higher frequency of occurrence of pre analytical error in the Inpatient department samples than outpatient and emergency samples here observed. Conclusion: Corrective actions based on the outcome of Pre Analytical Quality Indicators will be beneficial for patient care service. Adequate training is warranted in laboratory service.