Introduction: Hepatitis B Viral infection is a serious threat to dialysis patients. The prevalence of HBV is more among hemodialyzed (HD) patients than the general population and these patients have a tendency to become chronic carriers of HBV due to defective immune system. Aim and Objectives: The aim of this study was to identify the non responders to the Hepatitis B vaccine in chronic renal failure patients on hemodialysis. Materials and Methods: Blood samples were collected from seventy seven patients on hemodialysis and they were screened for HBsAg and Anti–HBs titres. Results: Of the total 77 haemodialysis patients 18 (23.37%) were females and 59 (76.63%) were males. Of the 77 patients 40 were non responders (Anti HBs Ag titre <10 IU/ml), 16 were poor responders (Titre between 10 – 100 IU/ml) and 21 were good responders (Titres >100IU/ml). Conclusion: As the nonresponders among the dialysis patients is high and the antibody titre falls with time in these patients, regular monitoring of Anti–HBs is necessary and booster doses are to be administered whenever necessary.