BACKGROUND: Hand hygiene, is the act of cleaning hands for the purpose of removing soil, dirt, and micro organism. Medical hand hygiene refers to hygiene practices related to medical procedures. Hand washing before administering medicine or medical care can prevent or minimize the spread of disease. Since Interns are in constant relationship with patients they play a paramount role in proper execution of hand hygienic practice among health personal. OBJECTIVES: To assess the knowledge of hand hygienic practice among medical professionals and to find out their awareness about hand hygienic practices. MATERIALS AND METHODS: A cross sectional study was conducted at sree mookambika institute of medical sciences among 2013 CRRI batch .RESULTS: Among the study population of 75, 33 were males and 42 were females.46% interns routinely use handrub,42% interns believe the frequent source of germs is hospital air,56% of study population practice hand hygienic methods before touching the patient to prevent transmission of germs to patient , 53% of study population practice hand hygienic methods after the exposure of body fluids to prevent transmission of germs to health care workers CONCLUSION: Hand hygienic practice protects and also prevents from infections. Among the study population though the knowledge about the hand hygienic practices are good, but they are not following in day to day practice.