Back ground: Yemen is classified as a low prevalence country with an estimated HIV prevalence at 0.3% in the general population. By the end of December 2018, a total of 10300 cases were reported to National AIDS Program. Objective: This study aims to determine HIV typing among Yemeni AIDS patients, and the association of possible risk factors. Methods: A cross–sectional study was conducted with 194 HIV–infected patients (134 males and 60 females) attending National AIDS Center at Aljumhory Educational Hospital, Sanaa, Yemen. Blood samples of HIV patients were collected, separated and tested for detection of HIV–1 and HIV–2 antibodies using commercially available kits of ELISA method. Results: Higher incidence rate of HIV–1 among male was 0.28/1000, more than female 0.11/1000. There were no effect of blood transfusion and contracting HIV–2 (P = 0.6), history of surgical operation (P =0.25), dental procedure (P = 0.6), or with history of traveling abroad (P =0.06). Highly significant of possible associated factors were among family member suffering from AIDS infection contracting HIV–2 and history of injury (RR=2.1, P= 0.01), (RR=2.4, P= 0.007) Conclusions: Majority of the HIV positive subjects were HIV–1 followed by HIV–2. Co–infection by HIV–1/HIV–2 may lead to antiretroviral resistance. HIV screening in Yemen should be test for co–infection; antiretroviral resistance