Individual and community health is one of the major goals of public health. Preventive medicine is focusing on diseases prevention and health promotion to maintain population’s health. The target of these services is to prevent onset or delay complication and progression of the disease. Periodic health examination (PHE) is a regular visit to the primary health care centers, where patients can receive preventive health services. Aim: to explore teacher’s knowledge and practice of periodic health examination in Al Khobar and Dammam, Eastern province. Method: Analytical cross–sectional descriptive study which was conducted at Eastern Province in Kingdome of Saudi Arabia (Dammam and AL Khobar). The study populations are Saudi female teachers of public schools in all levels. Data was collected by written Self–administrated questioner. Results: Our study conducted on 376 female participants. Most of participating teachers (54.5%) were from Dammam. Around half (50.8%) of the teachers age were between 31 and 40 years. As regards psychological disorders awareness, about (52.9%) of teachers in the study agreed that a person should visit A physician if you have low mood or anhedonia. Social media was the primary source of knowledge PHE among (42.8%) of participants. Concerning the Family history of medical diseases, about (56.6%) had family history of DM2. As regard dyslipidemia, more than half of participants (71.3%) disagreed that its starting age is 21 years. 24% participants had good knowledge about PHE. The percent of PHE practice among participants is only 25%. it was found that the relationship between place of work and total knowledge was significant (P–value = 0.037) with around half of participants who had good knowledge (54.9%) worked in Khobar. A significant relationship (P–value = 0.048) was seen also between marital status and total knowledge with more than half of those with good knowledge (86.8) were married. The relationship between total knowledge and the number of children was significant (P–value = 0.015) . Conclusion: Awareness and practice among women in the study towards periodic health examination is low.