Infection with hepatitis B virus can cause both acute and chronic liver disease. The virus can be transmitted through contact with blood or other body fluids of an infected person. All over the world, approximately 257 million people have been infected with hepatitis B virus (HBV). In 2015, hepatitis B resulted in 887000 deaths, mostly from HBV – related cirrhosis or Hepatocellular Carcinoma. In present study we assess the Knowledge, Attitude and Practice of Hepatitis B Vaccination among the medical faculty of MGM Medical College and Hospital, Aurangabad, Maharashtra. The present cross sectional Observational study among the medical faculty of MGM Medical College, Aurangabad, India. The study was conducted during 1st Jan 2018 to 31st Oct. 2018. In the present study 160 medical faculties were enrolled. A pre–structured questionnaire was developed consisting of the participant’s socio–demographic characteristics, vaccination status, barriers for immunization and basic awareness regarding HBV transmission. Out of 160 participants, 86 (53.7%) were males and 74 (46.2%) were females. Maximum participants were from age group of 31–40 years (45.6%).134 (83.7%) participants had been vaccinated against HBV. Out of total 160 participants, 92 (57.5%) had good awareness score of Hepatitis B vaccination and only 3 (1.9%) participants had poor awareness. The continued efforts are needed to increase awareness and vaccination coverage among medical professionals. The importance of doing anti Hbs titre after complete vaccination needs to be imposed upon the medical professionals for protection against accidental needle stick injuries.