This study aims to evaluate and compare the effect of intraoral environment on tensile strength and modulus of elasticity of different alignment wires on Universal Testing Machine. Materials and methods: 144 wire samples were divided into 4 groups(Group I– IV). Group I = 0.014 inch stainless steel wire, Group II = 0.014 inch Nickel–Titanium wire, Group III = 0.014 inch Thermoelastic Nickel Titanium wire, Group IV = 0.016 x 0.022 inch Graded thermodynamic Nickel Titanium wire, keeping 72 wire samples as Unused wire(A) and 72 as Retrieved(B) wire samples . Results: There was decrease in tensile strength and modulus of elasticity for Retrieved(B) wires.The tensile strength of Stainless steel was found to be maximum while Thermoelastic Ni–Ti showed minimal value. Modulus of elasticity of graded thermodynamic Ni–Ti showed minimal value. Conclusion: Graded thermodynamic Nickel Titanium wires were found to perform better clinically, delivering a constant force over time.