Background: Scrub typhus is one of the most covert emerging and re–emerging Rickettsial infections. In developing countries, among the various laboratory tests to diagnose scrub typhus, Weil–Felix test is commonly performed despite its low sensitivity. Objective: The present study was conducted to determine the seropositivity of IgM ELISA for scrub typhus in clinically suspected cases at tertiary care hospital. Methods: The study was a hospital based prospective study where 1915 clinically suspected cases of scrub typhus were tested by IgM ELISA using commercially available kits. Results: Out of 1915 sample tested, 633 (33.1%) samples were positive for Scrub typhus by IgM ELISA test. Out of total 633 seropositive samples 222 (35%) patients were male and 411(65%) were female patients. Conclusion: Scrub typhus should be included in the differential diagnosis of fever of unknown origin in Southern Part of Rajasthan by using IgM ELISA