Background: Pleomorphic adenoma (PA) accounts for about 60% of all salivary gland neoplasms (SGN). Material and methods: The case series consisted of 33 cases of PA received from the period between Jan 2008 and June 2014. IHC was performed by using primary antibodies were used, CK7,CEA, EMA, CD117, HMWCK, SMA, p63, CD10, Ki67 and p53. The immunoreactivity for all the markers except p53 and Ki67 was semi–quantitatively assessed in each case by estimating the percentage (%) of cells. Ki–67 Labelling index was recorded. The reaction for p53 was recorded as % of tumour cells showing nuclear staining with p53. Results: The ductal luminal markers CK7 – 20 (60.60%), EMA 14 – (42.42%),CD117–8(24.24%),CEA –5(15.15%) cases of PA showed intense immunoreactivity. The abluminal / myoepithelial IHC markers HMWCK – 14(42.42%), SMA– 21(63.63%), p63 – 9 (27.27%) and CD10 – 5 (15.15%) cases showed intense immunoreactivity. The prognostic markers such as Ki67– 11(33.33%) and p53 –19(57.57%) cases showed positivity. The highest Ki67 Li was 18% & 20%. Conclusion: The present study validates the understanding of the pathogenesis of PA. The IHC markers studied in the present article could help in diagnosing PA at unusual sites. The proliferative markers when done in PA helps determine the need for follow up in few cases assisting prognosis.