Objectives:1) To assess the existing knowledge regarding Hemophilia among teachers in school. 2) To evaluate the effectiveness of Self Instructional Module (SIM) regarding Hemophilia among teachers in school. 3) To associate knowledge score with selected demographic variables. Methods and Materials: The study was an evaluative approach conducted among 60 teachers from selected schools in Wardha. Structured knowledge questionnaire were used to collect the data. Results: Pre test knowledge regarding hemophilia in children among school teachers. 11(18.3%) of them had poor level of knowledge score, 43(71.67%) of them had average level of knowledge score, 4(6.67%) of them had good level of knowledge score, 2(3.3%)of them had very good level of knowledge score and none of them had excellent level of knowledge score. The minimum score was 0 and maximum score was 22, the mean score was 8.52 ± 4.280with a mean percentage value of 28.4%. Assessment of post test knowledge regarding hemophilia in children among school teachers. 2(3.3%) of them had average level of knowledge score, 1(1.6%) of them had good level of knowledge score, 22(36.67%) of them had very good level of knowledge score and 35(58.3%) of them had excellent level of knowledge score. Conclusion: Analysis of data showed that there was significant difference between pre test and post test knowledge scores. Hence it is concluded that the Self Instructional Module significantly brought improvement in the knowledge regarding hemophilia in children among school teachers.