Introduction: Present study, proposes immunohistochemical criteria for assessing structural and functional characteristics of Hassall’s corpuscles using antibodies against cytokeratin markers in human fetuses. Methods: Hassall’s corpuscles of 30 aborted human fetuses of various gestational ages (12 to 31 weeks) were studied by using CK5, CK8 and CK14 antibodies. Results: CK8 and CK14 positivity was seen around the periphery, while CK5 positivity was observed both at peripheral and central part of Hassall’s corpuscles. Two major growth spurts were observed after 16 and 22 weeks of gestation. Conclusions: This information will be helpful in enhancing the understanding of extent of similarity between the epidermal component and HCs and in designing effective molecular and cellular methods to treat both skin and immune disorders with the help of progenitor stem cells surrounding HCs within the human fetal thymic tissue.