Background: Malaria is the most important parasitic disease of mankind and caused by the protozoa parasite plasmodium. Malaria presents a diagnostic challenge to laboratories in most countries. Liver involvement in severe p.falciparum infection is commonly a significant cause of morbidity and mortality among humans LDH activity as an index in the monitoring of p.falciparum malaria infection, particularly when all other possible causes of increased serum LDH levels have been eliminated. Objectives: To study the LDH enzyme assay for the detection of p.falciparum severity in patients and to investigate the varying effects that different levels of parasite density have on blood cell count. Material and Methods: The present study was carried out on 104cases of falciparum malaria in the DTR Hospital Kurnool. A.P. Microscopic examination was used to count parasites. LDH activity was determined in patients with falciparum malaria. All these patients were subjected to blood investigation like Hemoglobin level RBCs count, WBC count and platelets count to know the severity of parasitaemia in children and adults patients. Results: A total 104 patients were enrolled. Out of the total number of cases 46 were children and 58 were adults. Irrespective of age LDH activity was significantly higher in the high level of parasitaemia patients. RBC count was significantly lower in 71(68%) of total patients with p.falciparum. leukocytes, neutrophil was higher in 65(63%) patients where as eoisinophil and monocyte count was lower in 59(57%) and 62 (60%) patients respectively. Platelet count was significantly lower in 72(69%) of total patients with p.falciparum infection. Conclusion: Serum LDH activity is potentially valuable enzymatic marker of acute p.falciparum malaria infection. The study finds correlation between levels of parasitemia and the activity of parasite LDH and also focuses on laboratory value in p.falciparum infected malaria with haematological parameters.