Comparative evaluation of Bolton’s ratio between males and females of Jaipur population. Subtitile: gender variation in Bolton’s ratio. Objective: This study was designed to find the gender variations in Bolton’s ratio in Jaipur population. Materials and methods: The study included two groups consisted of 150 male and 150 females’ casts. The measurement of mesiodistal dimensions of upper and lower tooth width was done using a digital caliper. Student’s t–test was to use to analyze the data with level of significance p ˂0.05. Statistical analysis was done using SPSS for windows software (version 21). Results: The overall ratio in males was 91.060 and in females were 90.996. The anterior ratio in males was 77.068 and in females were 76.468. There was no statistically significant difference between the groups. Conclusion: The Bolton’s overall and anterior ratio was similar for both males and females.