Type–2 Diabetes mellitus [T2DM] is a metabolic disorder characterized by chronic hyperglycemia with disturbance in carbohydrate, fat and protein metabolism. These metabolic abnormalities lead to long–term damage of various organs, causing their dysfunction and failure. Complications of T2DM are among the leading cause of mortality worldwide. The aim of the present study is to study and compare the lipid profile, serum amylase and UA in T2DM with controls. The present study was carried out in the department of Biochemistry of Rohilkhand Medical College, includes total 120 subjects: 60 known cases of T2DM and 60 age and sex matched healthy adult as control subjects. Based on the results of the present study and data available, it is clear that in Diabetes the serum level of lipid profile, UA and serum amylase were altered. This pilot study proves to be significant in respect of diabetes and diagnosis protocol.