Nigella sativa has protective role in Chronic Kidney Disease (CKD), hypertension and diabetes mellitus. We assessed effectiveness and safety of Nigella sativa oil supplementation in patients of hypertensive nephropathy in a comparative, prospective and open label study. Group 1 (Control) received conservative management while Group 2 (Test) received Nigella sativa oil along with conservative management of hypertensive nephropathy for 12 weeks. There was amelioration in clinical features and biochemical parameters but it was more noticeable in test group. A decrease in serum creatinine, blood urea, 24–hour total urinary protein (TUP) and blood pressure level was observed along with an elevation in glomerular filtration rate (GFR) and 24–hour total urinary volume (TUV). We confirm that supplementation of Nigella sativa oil is effective and safe in preventing the advancement of disease in patients of hypertensive nephropathy.