Background: Oral health and general health are interdependent and of the classic example of this symbiotic relationship is that of Periodontal health and the Diabetes mellitus. Diabetes mellitus, play a crucial role in the progression of periodontal inflammatory conditions. Alkaline phosphatase (ALP) enzyme plays a significant role in gingival inflammation and bone resorption. Aim: To compare the serum and salivary alkaline phosphatase levels in chronic periodontitis patients with or without type 2 diabetes mellitus. Materials and Methods: The study consisted of a total of 45 individuals which were allocated to three groups: Group I (healthy individual), Group II (Chronic periodontitis without diabetes mellitus type 2) and Group III (Chronic periodontitis with type 2 diabetes mellitus). The serum and unstimulated saliva were collected from all patients in aseptic condition and samples were analyzed for alkaline phosphatase level using AVANTOR™ Benesphera ALP Kit by fully automated analyzer. Results: The concentration of serum and salivary alkaline phosphatase were significantly high in patients with chronic periodontitis with type 2 diabetes mellitus. Conclusion: Alkaline phosphatase can prove a valuable clinical diagnostic biomarker in periodontal diseases.