BACKGROUND The waste produced in the course of health–care activities carries a higher potential for infection and injury than any other type of waste.Therefore, it is essential to have safe and reliable method for its handling. With this background, the present study was conducted to assess the knowledge and practices of health–care workers regarding Bio–medical Waste Management. MATERIAL AND METHODS A hospital based cross sectional study was carried out among 100 health care personnel selected through stratified random sampling during September–October 2018 at government medical college CIMS, Bilaspur, Chhattisgarh. A predesigned, pretested questionnaire pertaining to biomedical waste management rules 2016 was used for data collection. SPSS 21.0 version software was used to analyze the data. RESULTS Out of 100 respondents consisted of 50 intern doctors, 25 staff nurses and 25 lab technicians, about 77% of health care personnel were aware about biomedical waste categories. Around 57% of the respondents knew about maximum storage time limit for untreated waste while only 43% had the knowledge regarding pre and post exposure prophylaxis. Around 64% of health care personnel were discarding sharp waste correctly in white translucent puncture proof containers while ampoules and vials were discarded by 69% staff in blue cardboard boxes. Safe injection practices and onsite treatment of waste were followed by 64% and 56% respectively. CONCLUSION Intern doctors and staff nurses were aware regarding biomedical waste management rules 2016 and its crucial aspect but nursing staff were better in the practical implications of the same while the lab technicians lagged behind in both. Intensive training programs at regular intervals with supportive supervision and continuous medical education are essential with special emphasis on pre and post exposure prophylaxis and time to time amendment of rules.