Background: Infection is a communal cause of morbidity and mortality in burn patients. Clinical diagnosis of sepsis in burn patients is problematic for several reasons. Objective: To find out the bacterial profile for post burn infection in pus and blood. And to evaluate the antibiotic sensitivity of organisms cultured and isolated. Methods: A retrospective study was conducted at Multi–speciality Hospitals Burn’s Unit at Gwalior, Madhya Pradesh. Fifty burn patients were investigated for bacterial profile of burn wound infections. Specimens were collected on 3rd and 7th day of burns in the form of wound swabs. Antimicrobial susceptibility test was done by Kirby – Bauer disc diffusion method. Epi–info (version 7.0) was used for analysis. Results: Gram negative organisms were found to be more prevalent. The most common isolate was Pseudomonas aeruginosa (P. aeruginosa) –38%, followed by Staphylococcus aureus (S. aureus) –26%, Klebsiella spp.–4%, and Escherichia coli –11%. Conclusions: Pseudomonas was the commonest cause of infection in burn patients followed by S. aureus. About 80% of the isolates showed multiple resistances. Regular antibiotic resistance test must be done for each patient in order to select a suitable antimicrobial agent.