Introduction: Prostate cancer is the most common cancer in men and responsible for 10% of all cancer deaths. Its incidence has been increasing because of increase in aging population and as a result of screening techniques like PSA (prostate specific antigen), Digital rectal examination and Ultrasonography. Diagnosis is established by doing TRUS (Transrectal Ultrasound) guided needle prostate biopsy in suspected cases. Tissue diagnosis often becomes difficult because of smaller biopsy samples or due to histopathologic mimickers of prostatic malignancy. These include chronic prostatitis, basal cell hyperplasia, clear cell hyperplasia, atypical adenomatous hyperplasia and sclerosing adenosis. These benign mimickers can lead to serious clinical, psychological and medicolegal consequences. Immunohistochemical markers are often used in these circumstances to rule out malignancy. Basal cell markers are most commonly used for Immunohistochemical evaluation of prostate. Basal cell markers are of 2 types; nuclear marker (p63 and cytokeratin{CK5/6}) and cytoplasmic marker (high molecular weight cytokertin 34Eb12). The lack of basal cell Layer staining along with simultaneous demonstration of a positive basal cell layer in adjacent unequivocally benign glands is the most important diagnostic hallmark of prostatic adenocarcinoma. Aim & Objectives: Study different patterns of benign mimickers of prostate adenocarcinoma. Couple it with use of immunohistochemistry(IHC) for accurate diagnosis and avoid false positive interpretation. Material and methods: From Dec 2013 to Dec 2017, a prospective study was carried out in the department of pathology, SCB medical College, Cuttack. TRUS guided prostate biopsy samples were collected from 193 cases. A detailed histopathologic and immunohistochemical analysis was then carried out. Results : Routine Hemotoxylin –Eosin (H/E) stained sections showed BPH in the majority 92(47.67%), followed by suspicious of carcinoma in 32(16.6%) and overt carcinoma in 69(35.7%) cases respectively. Immunohistochemical analysis was done in suspected cases of adenocarcinoma using p63 and 34bE12. Results of IHC were prostate adenocarcinoma (n=8), chronic prostatitis (n= 11), basal cell hyperplasia (n=6), clear cell hyperplasia (n=3) and sclerosing adenosis(n=4) Conclusion: Our study confirms the diagnostic utility of basal cell markers p63 and 34bE12 in differentiating benign mimickers from the malignant ones in morphologically suspicious cases of prostatic biopsy specimens.