Aim – To evaluate the effect of the various commercially available desensitizing agents on the retention of full metal crowns cemented with different luting agents like, Glass Ionomer Cement, Resin Modified Glass Ionomer Cement and Resin Cement. Materials and Methods– 420 extracted maxillary premolars were collected and prepared using a paralleling milling device. The prepared teeth were divided into 3 major groups: GIC, RMGIC and Resin cement and into 7 sub–groups: Systemp, Gluma, GC Tooth Mousse, Pro–Arginine, Novamin, Flouride and control group. The desensitizing agents were applied before impression making, provisional cementation and permanent cementation. Wax patterns were fabricated and casted with a loop using Ni–Cr alloy. After cementation, half of the samples were subjected to thermocycling and then under tensional force the crowns were removed using a universal testing machine. Results– In Group I (GIC), the thermocycled samples showed highest retention with Systemp and least with Pro–Arginine and non–thermocycled samples showed highest retention with GC Tooth Mousse and least with Control group. In Group II (RMGIC), thermocycled samples showed highest retention with Systemp and least with Flouride and non–thermocycled samples showed highest retention with Systemp and Novamin group and least with Pro–Arginine. In Group III (RC), the thermocycled samples showed highest retention with Systemp and least with Flouride and non–thermocycled samples showed highest retention with Systemp and least with Pro–Arginine group. The retention was improved with thermocycling and Resin cement showed the highest retentive strength, followed by RMGIC and then GIC in all groups. Conclusion– Systemp, in all the subgroups showed the highest retention and Pro–Arginine showed the least retention. Resin cement showed the highest retentive strength. Also, thermocycling had increased the retention in all the samples irrespective of the type of cement used.