Diabetes mellitus is a syndrome of impaired carbohydrate, fat and protein metabolism caused by either lack of insulin secretion or decreased sensitivity of the tissues to insulin. Chronically elevated blood glucose level lead to metabolic derangement and as a result microvascular complication may occur as diabetic neuropathy. Nerve conduction study (NCS) is considered to be sensitive, reliable and objective means of investigating neuropathy. Among the various parameters used in nerve conduction study, F wave is the parameter known to be one of the late responses. It is used to know the status of proximal segment of nerve. In our study 100 subjects were included, 50 diagnosed cases of type 2 diabetes mellitus and 50 age and sex matched healthy subjects as control. We recorded F wave of nerve conduction study and then F minimum latencies (in milliseconds) were noted for both the diabetic and control group and compare it within these two groups. It is observed that F minimum latencies were prolonged in bilateral median, ulnar, peroneal and tibial nerves in type 2 diabetic patients compared to controls and differences were statistically significant(p<0.05). Prolonged F wave minimal latency in the tested nerves of upper and lower extremity bilaterally in diabetic group were suggestive of some sort of impairment in proximal segment of these nerve in diabetic group as F wave assess predominantly proximal segment of the nerve.