Background: Vitamin D is a secosteroid hormone and well–known for its classical actions in the maintenance of calcium uptake and bone metabolism. Recently, numerous in vitro experiments demonstrated that 1,25–(OH)2D3, the active form of vitamin D, inhibited the growth and differentiation of human prostate cancer cells. Aim: To estimate vitamin D level in prostate cancer patients along with increasing risk. Material & Methods : 100 samples along with control were analysed by cobas e–411 for Vitamin D and PSA. Results: we have found Higher risk of prostate cancer who having low vitamin D. Conclusion: it’s time to aware people for supplementation of Vitamin D, so we can prevent for the same