Introduction: Helicobacter pylori (H. pylori) chronically infect more than half of all people worldwide. We explore the rate of occurrence of H pylori in dyspeptic patients and its causal association with peptic ulcer disease, non–autoimmune gastritis, non peptic ulcer dyspepsia and gastric carcinoma. Objectives: To find out the rate of occurrence of H. pylori in dyspeptic patients and to emphasize the diagnosis of H. pylori by rapid urease test, serology, Giemsa stain of biopsy specimens and to correlate all these diagnostic tests with biopsy. Methods: An observational prospective cohort study was performed on 60 selected cases. Detail clinical history, laboratory tests and histopathological examination were done. Findings were recorded and analyzed statistically. Results: The comparison of patient’s age group, socio–economic status and association of H. pylori showed statistically significant. Dyspepsia was more common in male (68.3%) and the sex ratio was 2.15:1. The rate of occurrence of H. pylori in dyspeptic patients was 68.33% and for functional dyspepsia was 61.29%. Majority of dyspeptic patients were positive for IgG ELISA test, 65% were positive for the biopsy urease test and 55% in modified Giemsa stain for H. pylori in tissue sections. Incidence of chronic superficial gastritis, benign gastric ulcer and duodenitis/duodenal ulceration were noted and association with H. pylori was statistically significant. Conclusion: Early diagnosis may prevent dyspepsia and complications of H. pylori infection like chronic superficial gastritis, benign gastric ulcer, duodenitis/duodenal ulceration even adenocarcinoma.