Background: Myocardial infarction (MI), commonly known as a heart attack, occurs when blood flow decreases or stops to a part of the heart, causing damage to the heart muscle. The myocardium receives its blood supply from the two large coronary arteries and their branches. Occlusion of one or more of these blood vessels (coronary occlusion) is one of the major causes of MI.[1] Chest pain is the most common symptom of acute myocardial infarction (AMI) and is often described as a sensation of tightness, pressure, or squeezing. The pain associated with MI is usually diffuse, does not change with position and lasts for more than 20 minutes.[2] If an individual experiences chest pain that does not meet the criteria to be called as a classic anginal chest pain is termed as atypical chest pain (ACP). The symptoms experienced in such cases may be both cardiac as well as non–cardiac related. An anginal chest pain is a squeezing or pressure like sensation in the chest that is felt generally when an individual does any strenuous activity. This symptom is the classic presenting feature of MI. An atypical chest pain does not occur in the sternum and may radiate to other regions of the body as well. This pain is sudden sharp and short lived. Material & method: This study was conducted at Department of Biochemistry PBM hospital in association with Department of Cardiology– HRMC, Sardar Patel Medical College Bikaner, Rajasthan from april 2018 to march 2019. There were 50 study and 50 controls in the age groups from 20 to 70 years. Fresh samples were taken and required tests were performed following standard protocol. Troponin–I level was estimated by two–site ELISA method and CK–MB level was quantitatively estimated on fully auto analyzers using Immunoinhibition/ UV Kinetic method.[3] Result: Troponin–I showed high significance with p–value < 0.0001***. For CK–MB p–value was 0.0001** Conclusion: In this study the role of cardiac biomarkers– Troponin–I and CK–MB has been explored. Troponin–I is the most significant and preferred cardiac biomarker for early detection of MI.