Endometriosis is one of the major gynecological disorder affecting women in their reproductive age. The major cause of endometriosis is still unknown and there no proper noninvasive marker to diagnosis the disease at early stage. The aim of this study was to investigate the role of Human neutrophil peptides 1, 2 and 3, cytokines and matrix metalloproteinase’s in pathogenesis of endometriosis. The present study characterizes the detection of HNP1–3, IL–6, IL–8, MMP–7 and MMP–9 in serum may provide novel non–invasive markers and diagnostic test to identify different stage of endometriosis