Background: A woman can be diagnosed with gestational diabetes when glucose intolerance continues beyond 24 to 28 weeks of gestation. 1 in 3 women with diabetes were of reproductive age. 21.3 million of live births had some form of hyperglycemia in pregnancy. Methodology: This study was carried out in Department of Biochemistry and conducted on 50 clinically positive diagnosed women with GDM in their 2nd trimester of pregnancy represented as study group and 50 healthy pregnant women clinically negative diagnosed with GDM during the 2nd trimester as control group aged between 20–35 years attending Antenatal clinic part of Department of Gynecology and Obstetrics. Serum Albumin and Uric Acid were estimated. Result: Mean serum albumin level was decreased to 3.418gm/dl and mean serum concentration of uric acid was increased to 4.30mg/dl as compared to control group(3.5gm/dl & 3.03mg/dl). Conclusion: In this study, we found that gestational diabetes had significantly increased concentration of uric acid and a significantly decreased concentration of albumin than healthy pregnant women. Hyperuricemia seen in GDM could be caused by the effects of insulin on the kidneys. Decreased albumin level in insulin resistant subjects aggravates cardiovascular complications.