BACKGROND Invasive burn wound sepsis is the leading cause of death in burn victims. Antibacterial susceptibility patterns for micro–organisms isolated from the hospitalized burn patients are continuously evolving. METHODS A cross sectional study was performed by analysing consecutive, nonrepeat clinical samples of all burn patients admitted in a tertiary care referral burn centre and teaching hospital, over a period of one year. All patients were initially resuscitated and optimized on standard management protocols. Patients were actively followed up, and relevant samples (wound swab, blood and urine) from burn patients were collected aseptically. Samples were examined by standard microbiological techniques. RESULTS Out of a total of 81 isolates, 33 were Pseudomonas aeruginosa (40.74%) as the most frequent isolate, followed by 21 Acinetobacter (25.92%), 15 Klebsiella pneumoniae (18.51%), 4 Staphylococcus aureus (4.93%), 4 Enterococcus facecium (4.93%), 1 E. coli (1.23%), 1 Staphylococcus hemolyticus (1.23%), 1 Burkholederia cepacia (1.23%) and 1 Proteus mirabilis(1.23%). Positive cultures were significantly more frequent among patients with over two weeks duration of burn wounds. We found a greater number of positive isolates in the second week of burn duration (n=28;35% of total isolates). Maximum number of isolates were susceptible only to colistin and tigecycline (n= 68; 84%). We found that most of the organisms were resistant to rest of the antibiotics. CONCLUSION Psuedomonas, Acinetobacter and Klebsiella were the commonest isolate in burns at our centre, which were more frequent with over two weeks of burn duration. Colistin and tigecycline was the antibiotic sensitive to most of the isolates.