Objectives: To frame individualized gender – specific prediction equations for shuttle run distance using 20 metre shuttle run test in healthy, Indian children aged between 7 to 19 years and to correlate shuttle run distance with age, height, weight & body mass index. Methods: 494 subjects between the age group of 7 to 19 years were recruited in this study. Demographic details like age, gender, height, weight and body mass index were noted. 20 metre shuttle run test was performed and shuttle run distance was calculated for all participants. Results: Shuttle run distance significantly correlated with age (r =0.303), height (r =0.357), weight (r =0.294), and body mass index (r =0.126). The gender specific reference equation generated forboys is –367.724 + (27.225 x Age) + (487.457 x Height) – (3.213 x Weight) and for girls is 153.689 +(10.306 x Age) +(118.113 x Height) – (1.421 x Weight). Conclusion: The established prediction equation can be used as a reference to evaluate exercise capacity for children and adolescents and to improve the applicability of the 20 metre shuttle run test in clinical practice.