Certain morphological changes occur in various organs of the body of preeclamptic women like in kidney there is decreased glomerular filtration rate. Cystatin–C is a sensitive marker to estimate glomerular filtration rate (GFR). The aim of this study was comparison of serum level of Cystatin–C in third trimester of normal pregnancy without preeclampsia and pregnancy with preeclampsia. In this study, 250 normal pregnant females without preeclampsia and 250 cases of preeclampsia were evaluated. They were compared for serum Cystatin–C. Preeclamptic pregnant females showed a highly–significant relationship in serum Cystatin–C levels when the results were compared with normal pregnant females without preeclampsia.