Nirgundi a medicinal shrub available in two colour variants ie Krishna (Vitex negundo var. purpurascence ) and Shweta(Vitex negundom var. negundo). Medicinally this is said to be best antimicrobial and analgesic. Materials and Methods: Matured aerial parts of these two plants were collected and evaluated for macro–microscopic, physicochemical, phytochemical and antimicrobial activity. Results:Microscopic and physico–chemical study of these two test drugs has not shown any variation. Phytochemical study revealed Saponins exclusively at Vitex negundom var. negundo. Methanloic extract of two types of test drugs have shown zone of inhibition for both samples against S. aureus, S. Pyogenes, P. aeruginosa, E. coli.