The basic principles of Ayurveda provide a foundation for the maintenance of health. Human body has seven Dhatu and is composed of microscopic cells. Though all these Dhatu have their specific location in the body, however seventh Dhatu Shukra are present in each and every cell. In Ayurveda, there is well described about Shukradhatu Sarata which is responsible for production of new generation. Aims and objectives: To study of Shukra Dhatu Sarata–Asarata in females with respect to ovulation period and compare the ovulation period in Shukra Dhatu Sara and Asara females group. Methods: ethical committee approval and subjects consent obtained. Random Selection of 60 individuals and categorized into 2 groups –Shukradhatu Sarata (30 individual), and Shukradhatu Asarata (30 individual) with the help of proforma as described in MUHS. Ovulation prediction kits are used for determination of ovulation period in every month upto 6 month. Observation and results: Comparison between Shukradhatu Sarata and Asarata group in 1–5 month was statistically significant (p–value 0.038) and 1–6 month was statistically highly significant (p–value 0.006) that means Shukradhatu Sarata and Asarata group has strong relation with ovulation period. Conclusion: If we improve the quality of Shukradhatu can improve the health and status of reproductive system.