Background: Infection of sterile body sites has profound clinical implications and poor outcomes. Hence early diagnosis and prompt management is the key to reduce morbidity and mortality in these patients. Objectives: To study the bacterial agents responsible for sterile site infections along with their antimicrobial sensitivity pattern. Methods: A total of 100 body fluids were subjected to cultural analysis according to standard laboratory procedures. Antibiotic sensitivity testing was done by Kirby Bauer Disc diffusion method as per CLSI Guidelines. Results: Culture positivity rate was 45%. Pleural fluids accounted for 44% of the total, followed by ascitic fluids (25%). A positive culture was seen in 72% and 52.6% ascitic fluids and peritoneal fluids respectively. Gram negative bacilli were isolated in 71.1% culture positive cases, among which, Klebsiella pneumoniae accounted for 46.9%. Antibiogram pattern showed 72.4%, 65.7% and 65.7% sensitivity, respectively, to Colistin, Amikacin and Carbapenems among Gram negative bacilli and 100% sensitivity to Vancomycin in Gram positive cocci. Culture positive patients were predominantly in the age group 31–40 years (42.2%). The male : female ratio was 1.4 : 1. Conclusion: Timely and appropriate antibiotic treatment; often empiric, can improve the clinical outcome of body fluid infections. Hence, knowledge of bacterial pathogens and their antibiogram pattern, prevalent in a locality, needs to be available and updated on a regular basis.