Aim: To compare the antimicrobial efficacy of Diode laser and Photo Activated Disinfection(PAD) against E.faecalis. Methodology: Thirty extracted premolars were instrumented upto Protaper rotary file size F2 and contaminated with a known species of E.faecalis .The samples were Group 1:PAD and Saline; Group 2:Diode laser and Saline; Group 3: NaOCl and PAD; Group 4:NaOCl and Diode laser; Group 5: Positive control. The samples were incubated in 37◦C for 48 h in BHI agar and colony forming units were counted. Statistical analysis: Descriptive analysis for CFUs as Mean & SD. Kruskal Wallis Test & Mann whitney Post hoc Analysis for comparison between groups. Results: Diode laser with NaOCl and PAD with NaOCl showed similar reduction in the growth of E.faecalis (P ≤0.001). Conclusions: A combination of NaOCl irrigant activated with Diode or PAD showed similar outcome. PAD can be considered as an alternative to Diode Laser for root canal disinfection.