Background – Lipid Profile significantly affects lipoprotein metabolism as well as some cardiovascular disease (CVD) so considered as cardiovascular risk factors.[1,2] A linear increase in total cholesterol (TC), low–density lipoprotein cholesterol (LDL–C) and triglycerides (TGs) and a linear decrease in high–density lipoprotein cholesterol (HDL–C) levels has been observed with increasing cardiovascular risk [3]. Routinely Fasting lipid Profile is advised for estimation but everytime it is not possible for patients to remain fasting and also due to overburdeon for analyst during morning hours during collection of fasting sample. So, aim of this study is to compare & correlate between fasting & nonfasting lipid profile values (any variation in lipid profile results) which are risk factors for Cardiovascular diseases.[4] Materials & Methods – This study include about 200 patients attending the outpatient department of clinical Biochemistry, IGGMC, Nagpur. All patients who were attending biochemistry OPD for Lipid Profile tests were selected for this study. History was taken as per designed proforma and consent form was obtained. Their Fasting Blood samples was analysed for lipid profile as well as random sample also taken & estimation of Nonfasting lipid profile test ie Serum cholesterol, Triglycerides, HDL,LDL & VLDL in clinical Biochemistry Laboratory was done. All these tests was run on Autoanalyser EM 460. Serum values of Both Fasting & Non fasting lipid profile was obtained and values were compared & correlated. The data was analysed & Student‘s T–test was used for the correlation of values . P <0.05 was considered significant. Result – Our results shows there is no statistically significant difference between fasting & Non fasting Serum lipid profile tests ie serum cholesterol level as well as serum Triglycerides,LDL and VLDL level , HDL shows not much difference in fasting & nonfasting levels .So, Nonfasting ie Random lipid profile tests can be done specially when it is inconvenient for patients to come empty stomache & also to reduce overburdeon on laboratory during morning hours. Conclusion – Results of our study indicates how the parameters of risk factors for coronary heart disease ie lipid profile including Serum total cholesterol, Triglycerides, HDL, LDL,VLDL doesn’t show any statistically significant difference in fasting & nonfasting values , so collection of sample in morning or random (nonfasting) doesn’t make much difference in their prediction in diagnosis of cardiovascular risk.