DIF studies are done for the detection of tissue bound antibodies in immune–mediated skin diseases. This study aimed to assess the role of DIF in diagnosing the dermatological diseases by correlating the clinical, histological and DIF diagnosis. DIF testing was done on the biopsy samples taken from 150 suspected cases of autoimmune diseases, connective tissue diseases and vasculitis along with the histopathological examination. The overall positivity of DIF in immune–mediated skin diseases was 81.3% in this study. The sensitivity of DIF was 94.1% in the pemphigus group and 88.5% in pemphigoid group. A positive lupus band test was noted in 17/23 cases of lupus erythematosus. DIF was positive in 10/15 cases of dermatitis herpetiformis. All the suspected cases of vasculitis were confirmed by DIF studies. False–negative results were seen.DIF is very helpful for the definitive diagnosis in autoimmune blistering diseases with clinico–histological dilemmas.