Introduction Hepatitis B (HB) is a serious global public health problem that causes chronic liver disease. Aims and objectives the aim of this study was to evaluate knowledge, attitude and practice (KAP) towards HB among dental students of Darbhanga. Materials and methods A KAP study was conducted in which a pre–validated WHO questionnaire was modified for the purpose of this study. A total of 113 students participated in this study. Result Mean knowledge, attitude and practice score for the entire study was 12.83 + 1.631, 4.35 + 0.989 and 6.35 + 0.778 respectively. Conclusion Dental students reflected fairly moderate level of KAP regarding HB infection and vaccination but still multiple gaps in knowledge and misconceptions in attitude and practices were identified.