The study was conducted with the objective to assess the awareness regarding hand hygiene among health care workers of Associated hospital of GMC Kathua. A cross sectional study was conducted at Associated hospital of GMC Kathua for two months from September to October 2019. Prior permission was taken from Institutional Ethical Committee. Primary data was collected by interviewing health workers of different designation from different sections. 50 % of the staff was selected randomly by lottery method. WHO Standardized questionnaires for hand hygiene was used as Study tools. Questionnaire1 comprised of the knowledge component among health care workers and the second questionnaire comprised of 5 questions from ‘My 5 Moments of Hand Hygiene ‘by WHO for assessing the knowledge and practice of Health Care workers during working hours. Data was entered in Microsoft excel. Descriptive statistical analysis was expressed in form of number and percentages. Total of 111 participants responded which includes 20 doctors, 40 staff nurses, 21 lab technicians, 10 FMPHWs,8 Sweepers and 12 others. 45% of participants received formal training in hand hygiene.81.18% of respondents used alcohol based hand rub. There was significant difference between knowledge and practice of My 5 moments of Hand Hygiene. Majority of Health Care Worker were knowledgeable but they had poor practice regarding the hand hygiene.