Objective: To know the anti–infective effectiveness of ascorbic acid, after a linear incision in the mucosa of the mandibular antero–inferior segment of the oral cavity of the guinea pigs, housed in the vivarium of the Biology Center of the Central University of Ecuador. Materials and methods: This study is experimental in–vivo and longitudinal, where an incision was made in the mucosa of the mandibular antero–inferior segment of the oral cavity in guinea pigs, three groups of 10 guinea pigs were formed, Group C, received mega doses ( 1.42 ml / 214.2 mg) which contained high amounts of ascorbic acid, Group B received normal doses (0.095 ml / 14.25 mg) and group A or control group did not receive ascorbic acid, this dose was applied one day before, during, and one day after the incision. The clinical evaluation was performed at 24 and 72 hours post incision, a sample was taken by swab from the wounded area at 72 hours for the planting of bacterial cultivation. Results: The clinical examination shows that at 24 hours in group C, 0% bleeding, edema, and erythema, and 100% epithelial junction, while in group B, 30% bleeding, 50% edema, 50% erythema and 100% epithelial junction and in group A 50% bleeding, 40% edema, 40% erythema and 20% epithelial junction; at 72 hours the results in group C and B showed better healing percentages in relation to group A, in this group some guinea pigs still showed bleeding edema and erythema in the wounded area. Conclusion: It is determined that the guinea pigs who received mega doses of ascorbic acid presented a better result in the process of healing after clinical examination at 24 and 72 hours and the swab exam shows a lower amount of bacterial colonization, thus demonstrating the effectiveness of ascorbic acid in mega doses of infectious processes.