In accordance with the Government of India Initiative of Swatch Bharat Mission 2017 and Guidelines for Management of Healthcare Waste as per Biomedical Waste (BMW) Management rules 2016, technical requirements of waste handling need to be understood and practiced by each category of the Health Care Worker (HCW). Importantly BMW poses grave risks to the health care providers, patients, community in general and the environment in a larger picture. Objectives: Assessment of Bio–medical waste management knowledge, attitude & practices (KAP) including segregation, collection, in–house transportation, pre–treatment, storage and disposal at various secondary and tertiary health care centers of Bhopal. Assessment of safety practices including vaccination and personal protection equipments (PPE) for the HCW involved in Bio–Medical Waste Management. Materials and Methods: Simple randomised questionnaire based cross sectional study done during Jan–June2019. Standard questionnaire was framed and distributed randomly among the respondent HCW. Conclusion: Better implementation of the revised BMW management guidelines; continual motivation; and training & retraining is a must to maintain the standards of bio–medical waste management.