Background Antimicrobial resistance is one of the most important threats to human health. Multiple surveillance programs have been launched worldwide to monitor the spread of resistant strains in community acquired and noncosmial infections. Urinary tract infection (UTI) is the second most common bacterial infection managed in primary care and Escherichia coli is the most common pathogen causing UTI.5,6) Selection of empiric antibiotics for urinary tract infections (UTI) has become more challenging because of the increasing rates of multidrug resistant organisms especially multidrug resistant enterobacteriaecae(MDRE) mostly ESBL’s and carbapenemases .(1) Enterobacteriaecae are the most common cause of urinary tract infections (UTI) in both community and healthcare settings. Selection of empiric antibiotics for UTI’s is often therefore based on the local or institutions susceptibility profiles. MATERIALS AND METHODS:– The study was conducted among the different age groups of both males and females attending at KPC Medical College, Jadavpur, Kolkata during two month of period included in this study. A total of 100 urine samples received from patients of the ICU of the medical college & hospital. All urine samples were processed within 1 hour after collection for aerobic bacterial culture. RESULTS:– 1. Bacterial isolate specimens and population characteristics:– A total of 50 E. coli isolates were obtained from the collected urine samples, of which 19 isolates were derived from men and 31 E. coli isolates from women. Forty –seven (94%) of the 50 E. coli isolates were resistant to at least one of the tested antimicrobial agents. Thirty–five (70%) of the 50 E.coli isolates were resistant to more than one antimicrobial agent. Out of 100 urine specimens processed in this study, 75(75%) showed significant bacteriuria. This study was done in the period June 2018 to August 2018 in KPC Medical College &hospital. CONCLUSION:– Urine infection is one of the most common medical problems affecting the population. This retrospective study was performed with the aim of surveying the incidence of antibiotic resistance patterns of bacterial agents, isolated from patients with UTI–positive cultures between June 2018 and august 2018 in KPC medical college & hospital Kolkata. In the present study, 75 patients with UTI–positive cultures were identified, 50 cases (66.6%) were female and 25 cases (33.3%) were male.