Renal allograft biopsy is the gold standard for diagnosis of rejection. Incorporation of C4d as a marker for humoral rejection is a major addition for Banff Schema, 2005. We evaluated the pattern of C4d staining in indicated renal allograft biopsies from January 2009 to March 2012 in Ruby hall Clinic, Pune. There were total 108 renal allograft biopsies received in the department; out of which 59 biopsies had features consistent with rejection. C4d is the degradation product of the activated complement factor C4, a component of the classical complement cascade which is typically initiated by binding of antibodies to specific target molecules. C4d staining was compared by two methods– Immunofluorescence and IHC. The intensity of expression of C4d in formalin–fixed paraffin–embedded tissues byIHC (P–IHC) was significantly reduced in comparison with frozen sections analysed by immunofluorescence (F–IF).