Background&objectives: India has the second highest HIV population in the world with about 2.5–3.0 million cases.Out of this 1.27 cases have been reported from Gujarat. Cases among general and blood donor population have also been reported mostly from north–western and southern states of India. This single centre study was carried out to observe the HIV reactivity among blood donors from Rajkot. Methods: A total of 20,395 donors were screened for the presence of HIV infection over the 1–year period. All tests were done using the fourth generation MERELISA ELISA kit which detected the presence of HIV–1 & HIV–2 P24 antigen and anti–HIV antibodies. Results: A total of 22 (0.12%) donors were found to be repeat reactive for HIV. Of these all were males. Out of this 5 (22.7%) were nonreactive on rapid tests. Interpretation&conclusions: The prevalence of HIV was 0.12% per cent among blood donors of PDU medical college, Rajkot.