Introduction: Chronic suppurative otitis media (CSOM) is a destructive and persistent disease with irreversible sequel and can proceed to serious intra and / or extra cranial complications. Causative agents of infection include bacteria, fungi and viruses with bacteria being the commonest cause. Hence bacteriological studies of CSOM are important for determining effective antibiotic choice and surveillance of bacterial patterns and their relative sensitivities. Materials and Methods: Total 157 patients having CSOM were included in the study. Ear discharge was processed with standard Microbiological procedures. Results: On aerobic culture Staphylococcus aureus 58 (36.96%) was the most common isolate followed by Pseudomonas aeruginosa 35 (21.21%). Fungal culture yielded 19 (11.2%) isolates. Conclusion: Evaluation of microbiological pattern and antibiotic sensitivity of isolates is necessary to decrease the potential risk of complications of CSOM by early institution of appropriate treatment.