BACKGROUND: The lungs are paired essential organs of respiration located within the thoracic cavity. The Knowledge of the position and grade of accessory fissures is essential for appreciation of lobar anatomy. OBJECTIVE: To study the morphological anatomy of accessory fissures in human cadaveric lungs and its surgical importance. MATERIAL AND METHODS: The study was conducted on 30 pair of human cadaveric lung specimens (30 left and 30 right) of unknown age and sex of Rajasthan population from the collection of Department of anatomy of Mahatma Gandhi Medical College & Hospital Jaipur. RESULT: Out of 30 pair of lungs, superior accessory fissure on left lung found 6.66% and inferior accessory fissure observed on left side 3.33% and right side 6.66%. transvers accessory fissure was observed only on left side in 13.33% cases. CONCLUSION: Awareness about accessory fissures is essential for differential diagnosis, segmental resection and performing lobectomies.