Liver is the largest abdominal viscera. In mammals it is an important organ which performs a wide range of vital functions such as metabolic activities, immune defense as well as important for nutritional value. Liver performs all above function in animals also but the morphology of this organ is slightly deferent in animals which can some idea about evolutionary steps from cattle to human. The present study is focused on Morphology of liver of Human, cow, goat and sheep manually because of expensiveness and unavailability of different technique like MRI, CT scan etc. The study was carried out in Dept. of Anatomy, Hi–Tech Medical College and Hospital, Health Park, Rasulgarh, Bhubaneswar. Total twenty liver specimens have been taken for study out of those five of each of Human, Cow, Goat and Sheep were included in study. All specimens were of deferent age groups and sexes. All the specimens were fixed in formalin and finally analyzed morphologically. The results show variability in organ’s position and it’s presenting parts. There was variation in morphological structure between human and animals. The study will helpful to explore the evolutionary changes and will also enlighten the knowledge of veterinary doctors.