In–vitro fertilization is one of the modern treatment modalities to treat infertility but it may induce some risk factors like multiple births, premature delivery and low birth weight, birth defects etc. Moreover recurrent IVF procedures may cause financial, physical and emotional stress. But the parents of IVF children are more concerned to their beloved child. So oral hygiene cares are one of the most important parts of their life style. Tooth brushing habits become established during the first years of childhood and last throughout lifetime. Aims : Aim of the study is to compare the tooth brushing habits of IVF children and spontaneously conceived children of same age group. Methods : A random sample of 2 – 5 years old IVF children and spontaneously conceived children were included in the study. Duration of brushing frequency and frequency of brushing assistance both group of children were analyzed with statistical analysis. Results : Statistically significant relationship was found between two groups in some arena of study. Conclusion : Through the obtained data, the present study shows that IVF children are better than spontaneously conceived children in the studied aspects.