Introduction – Methicillin resistant staphylococcus aureus (MRSA) is a bacterium that causes infections in different parts of body. It is difficult to treat most strains of Staphylococcus aureus because of their resistance to commonly used antibiotics. Materials and methods– This is cross sectional study of clinical and carrier MRSA sample of patients attending Integral Institute of Medical Sciences Research and Hospital Lucknow including IPD, OPD Patients and hospital personnel from January 2019 to June 2019. Result–In our study, the prevalence of MRSA in clinical samples was 66.7% and in carrier samples prevalence of MRSA was 65.6%. Antibiotic sensitivity once done for both types of clinical and carrier sample of staphylococcus aureus showed phenotypic relatedness in around 1.12 % of cases i.e., in four nasal swabs and rest of 98.8% cases of carrier MRSA. Overall antimicrobial sensitivity pattern in S.aureus in various samples showed 100 % sensitivity to vancomycin, teicoplanin, linezolid, amikacin, tobramycin, followed by tetracycline and doxycycline which had both 69.77%, clindamycin 65.12%, gentamycin and cotrimoxazole which had both 62.79% and other antibiotics showed lowest sensitivity in which erythromycin 18.60%, followed by levofloxacin 17.44%, nitrofurantion 12.79%, cefoxitin 10.47% and ciprofloxacin 9.30% respectively . Conclusion– Our study shows that if good infection control practices will be employed in hospitals ,health care and clinical institutions like proper cleaning ,proper waste management ,proper disinfection ,state of art ICU care and on top of all a good hand hygiene being practiced by the staff , then the rate of colonization and transmission of MRSA will decreases by much higher rate.