Aim: The aim of apical surgery is to eliminate periapical infection. An in vitro study was carried out in the Department of Conservative Dentistry &Endodontics, Ahmedabad, to evaluate the sealing effectiveness of Er:YAG laser, Ultrasonic Endo Retro Tip, and Round Bur in preparation of Root end Cavity using similar sealing material. Methodology: 52 tooth samples were divided into 3 groups: Laser group, Ultrasonic group, and Round bur group. Root canal treatment was performed on all samples. Then root ends were resected and root end cavities were prepared using different methods. Cavities were sealed by IRM and then all samples were kept in basic fuchsin dye for 48 hrs. Samples were sectioned longitudinally and were observed under stereomicroscope for the penetration of dye. Results: Laser group shows less microleakage(mean=0.96mm), Ultrasonic group shows more leakage( 2.14mm) and Round Bur showed maximum leakage(2.38mm) with laser group shows significantly less leakage than other two groups. But no statistically significant difference was found between ultrasonic and round bur group. Conclusion: In this reference, the results of the present study suggest that, Er:YAG laser treatment shows less microleakage compared to other two groups when used for retrograde cavity preparation.